Lab Facility: Mavericks (Room 155) Primary Trainer: Swaroop Kommera. The Zetasizer APS is a DLS instrument that automates the measurements of samples from industry standard 96 or 384 well plates. Malvern Dynamic Light Scattering (DLS) Zetasizer Training. In this application note, the measurements are repeated on a Zetasizer Automated Plate Sampler (APS) to demonstrate that its lower size limit is equivalent to the Zetasizer Nano S. Multi-Angle Dynamic Light Scattering (MADLS) improves the resolution of DLS. The diffusion coefficients and subsequent sizes obtained corresponded well with known structural data as well as diffusion coefficients of sucrose measured by other techniques. Accurate, reliable and repeatable particle size analysis in one or two minutes. These measurements were made on a Zetasizer Nano S instrument and proved that subnanometer sizes were possible by DLS. A sucrose molecule consists of a glucose joined to a fructose molecule via a linker and has a molecular weight of 342.3 Da.Ī previous study measured the size of sucrose solutions using DLS. Sucrose is an ideal candidate to test the performance of a light scattering system as it is small, easily obtained, available in a crystalline, pure form and readily dissolves in water. However, for sizes less than a nanometer, there are no proteins available. Proteins such as lysozyme are often used as test samples for sizes below 10nm. Traceable latex standards are only available down to 20nm in diameter. To explore the lower size limit of a dynamic light scattering (DLS) instrument, a suitable small, well defined particle or molecule that produces enough excess scattering needs to be used.
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